Pyrophosphate (PPi) played crucial roles in various fundamental physiological processes. Herein, a two-photon absorption (TPA) "On-Off-On" type benzoxazole-based fluorescence probe BN was designed and synthesized, which detected PPi through Cu displacing method in situ system in aqueous medium. The on-off-on process of BN recognizing PPi was verified by mass spectra and theoretical calculations, which was successfully applied in TPA cells imaging.
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| http://dx.doi.org/10.1016/j.talanta.2018.11.036 | DOI Listing |
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