AI Article Synopsis
- Hybrid strains are created by mating monosporic isolates or neohaplonts, which can be produced through chemical methods or protoplast techniques.
- Studies show that neohaplonts differ in growth rates and exhibit phenotypic and genetic changes based on the method used for their recovery.
- Analysis using six ISSR markers revealed 59.2% polymorphism among the neohaplonts, indicating substantial variation in both growth rates and genetic traits.
Article Abstract
Production of hybrid strains is accomplished by mating monosporic isolates or neohaplonts, obtained either by chemical dedikaryotization or by production of protoplast. However, differences in growth rate among recovered neohaplonts have been reported. The presence of phenotypic and genetic changes among the neohaplonts recovered either by chemical dedikaryotization or by production of protoplast, was evaluated by measuring growth and morphology, and by molecular characterization using six ISSR markers to identify polymorphisms. Neohaplonts recovered by both methods presented variation in growth rate depending on their compatibility type and recovery method. Using ISSR markers, 59.2% polymorphism was established. Neohaplonts recovered by both monokaryotization procedures presented differences in growth rate and polymorphism.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314949 | PMC |
| http://dx.doi.org/10.1007/s13205-018-1532-4 | DOI Listing |
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