Background: Oral cancer is a common cancer with a high mortality rate. While surgery is the most effective treatment for oral cancer, it frequently causes deformity and dysfunction in the orofacial region. In this study, methyl aminolevulinate photodynamic therapy (MAL-PDT) as a prevention tool against progression of precancerous lesion to oral cancer was explored.
Methods: For in vitro studies, we evaluated the effects of MAL-PDT on viability of DOK oral precancerous cells by XTT, cell morphology by TEM, and intracellular signaling pathways by flow cytometry, Western blotting, and immunofluorescence. For in vivo study, DMBA was used to induce oral precancerous lesions in hamsters followed by MAL-PDT treatment. We measured tumor size and body weight weekly. After sacrifice, buccal pouch lesions were processed for H&E stain and immunohistochemistry analysis.
Results: MAL-PDT induced autophagic cell death in DOK oral precancerous cells. The autophagy-related markers LC3II and p62/SQSTM1 and autophagosome formation in DOK cells were increased after MAL-PDT treatment. In vivo, Metvix -PDT treatment decreased tumor growth and enhanced LC3II expression in hamster buccal pouch tumors induced by DMBA.
Conclusions: Our in vitro and in vivo results suggest that MAL-PDT may provide an effective therapy for oral precancerous lesions through induction of autophagic cell death.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/odi.13036 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The study of elastic fibres in oral precancerous and cancerous lesions using Shikata's modified orcein stain: a retrospective study.
Ecancermedicalscience
November 2024
Department of Public Health Dentistry, DY Patil University School of Dentistry, Nerul Navi Mumbai, Sector 7, Nerul, Navi Mumbai 400706, Maharashtra, India.
Statement Of The Problem: Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer. During the invasion, tumour cells break through the basement membrane and penetrate the connective tissue to interact with the extracellular matrix. An attempt was made to evaluate the connective tissue changes in different grades of OSCCs, oral submucous fibrosis (OSMF) and Oral Epithelial Dysplasias.
View Article and Find Full Text PDFSmoking-induced shifts in salivary exosomal cytokines and amino acid profiles as potential early biomarkers for oral cancer.
Cytokine
January 2025
Centre for Medical Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, India. Electronic address:
Background: Chronic smoking is an established risk factor for oral cancer (OC). The role of tobacco in oral squamous cell cancer (OSCC) emphasizes the need for non-invasive diagnostic approaches to identify early molecular alterations and improve patient outcomes. Salivary exosomes, which contain proteins, lipids, and nucleic acids, accessible and rich in biological content, making them interesting candidate biomarkers.
View Article and Find Full Text PDFProspects for Narrow Band Imaging Magnification Endoscopy in Oral Lesions: Recommendations from Oral and Maxillofacial Surgeons and a Gastroenterologist.
Cancers (Basel)
December 2024
Division of Oral and Maxillofacial Surgery, Department of Oral and Maxillofacial Reconstructive Surgery School of Dentistry, Iwate Medical University, Morioka 020-8505, Iwate, Japan.
Narrow band imaging (NBI) magnification endoscopy for the diagnosis of early-stage oral cavity-related cancer and precancerous lesions can recognize oral lesions as brownish areas, and can observe intraepithelial papillary capillary loops (IPCLs) in the mucosa and submucosa to make a qualitative diagnosis of the lesion and highlight the mucosal surface microstructure to facilitate appropriate diagnosis and early treatment. IPCLs are classified from Type 0 to IV: Type 0 is normal mucosa or no blood vessels observed, e.g.
View Article and Find Full Text PDFSalivary proteomics and metaproteomics identifies distinct molecular and taxonomic signatures of type-2 diabetes.
Microbiome
January 2025
Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Blegdamsvej 3B, Copenhagen, 2200, Denmark.
Background: Saliva is a protein-rich body fluid for noninvasive discovery of biomolecules, containing both human and microbial components, associated with various chronic diseases. Type-2 diabetes (T2D) imposes a significant health and socio-economic burden. Prior research on T2D salivary microbiome utilized methods such as metagenomics, metatranscriptomics, 16S rRNA sequencing, and low-throughput proteomics.
View Article and Find Full Text PDFOral microbiome dysbiosis is associated with precancerous lesions and disorders of upper gastrointestinal tract: A population-based study.
Am J Gastroenterol
December 2024
Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, 171 77 Stockholm, Sweden.
Background And Aims: Oral microbiota may contribute to the development of upper gastrointestinal (UGI) disorders. We aimed to study the association between the microbiome of saliva, subgingival and buccal mucosa, and UGI disorders, particularly precancerous lesions. We also aimed to determine which oral site might serve as the most effective biomarker for UGI disorders.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!