During the last two decades, cross-linking combined with mass spectrometry (MS) has evolved as a valuable tool to gain structural insights into proteins and protein assemblies. Structural information is obtained by introducing covalent connections between amino acids that are in spatial proximity in proteins and protein complexes. The distance constraints imposed by the cross-linking reagent provide information on the three-dimensional arrangement of the covalently connected amino acid residues and serve as basis for de-novo or homology modeling approaches. As cross-linking/MS allows investigating protein 3D-structures and protein-protein interactions not only in-vitro, but also in-vivo, it is especially appealing for studying protein systems in their native environment. In this chapter, we describe the principles of cross-linking/MS and illustrate its value for investigating protein 3D-structures and for unraveling protein interaction networks.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-981-13-2200-6_8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Insights into NEK2 inhibitors as antitumor agents: From mechanisms to potential therapeutics.
Eur J Med Chem
January 2025
Department of Respiratory and Critical Care Medicine, Targeted Tracer Research and Development Laboratory, Institute of Respiratory Healthand, Department of Frontiers Science Center for Disease-related Molecular Network, Core Facilities, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China. Electronic address:
NEK2, a serine/threonine protein kinase, is integral to mitotic events such as centrosome duplication and separation, microtubule stabilization, spindle assembly checkpoint, and kinetochore attachment. However, NEK2 overexpression leads to centrosome amplification and chromosomal instability, which are significantly associated with various malignancies, including liver, breast, and non-small cell lung cancer. This overexpression could facilitate tumor development and confer resistance to therapy by promoting aberrant cell division and centrosome amplification.
View Article and Find Full Text PDFQuantification of Albumin and ɣ-Globulin Concentrations by Multivariate Regression Based on Admittance Relaxation Time Distribution (mrARTD).
Biomed Phys Eng Express
January 2025
Dept. Mechanical Engineering, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, Chiba, 263-8522, JAPAN.
Albumin and γ-globulin concentrations in subcutaneous adipose tissues (SAT) have been quantified by multivariate regression based on admittance relaxation time distribution (mraRTD) under the fluctuated background of sodium electrolyte concentration. The mraRTD formulates P = Ac + Ξ (P: peak matrix of distribution function magnitude ɣP and frequency τP, c: concentration matrix of albumin cAlb, γ-globulin Gloc, and sodium electrolyte Nac, A: coefficient matrix of a multivariate regression model, and Ξ: error matrix). The mraRTD is implemented by two processes which are: 1) the training process of A through the maximum likelihood estimation of P and 2) the quantification process of cAlb, Gloc, and Nac through the model prediction.
View Article and Find Full Text PDFA pilot project of pregnancy-associated plasma protein-A as an early screening test for preeclampsia in a Regional Hospital.
Gac Med Mex
January 2024
Departamento de Diagnóstico y Terapia Fetal, Centro Médico para Atención Fetal Especializada, Mexico City.
Application of the 6-SNP elevated LDL-cholesterol polygenic risk score in individuals with familial hypercholesterolemia phenotype from an Argentine population.
Gac Med Mex
January 2025
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica, Laboratorio de Lípidos y Aterosclerosis, Ciudad Autónoma de Buenos Aires.
Introduction: LDL-cholesterol greater than 190 mg/dL indicates severe hypercholesterolemia (HS) of monogenic and/or polygenic origin. Genetic risk scores (GRS) evaluate potential polygenic causes.
Objective: we applied a GRS of 6-SNP (GRS-6) in HS individuals.
The secrets of the Tübingen Castle kitchen: Friedrich Miescher and the discovery of nuclein, the cornerstone of DNA.
Gac Med Mex
January 2025
Departamento de Anatomía Patológica, Fundación Clínica Médica Sur; Departamento de Biología Celular y Tisular, Escuela de Medicina, Universidad Panamericana. Mexico City, Mexico.
In 1869, Friedrich Miescher, born in Basel, Switzerland, discovered a previously unknown phosphorus-rich substance in the nuclei of pus cells. Conducting his research in a laboratory set up in the kitchen of Tübingen's medieval castle in Germany, and under the guidance by Professor Felix Hoppe-Seyler, Miescher primarily focused on the composition of cell nuclei. He obtained nuclear material by washing pus cells from surgical bandages provided by a nearby hospital.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!