The article summarizes data of research studies concerning searching optimal conditions of development of highly specific and highly sensitive immune chromatography test-systems designed for detection of agents of infectious diseases. The analysis was implemented concerning choosing of optimal size of nano-particles of colloid gold and concentration of antibodies for production of conjugate. The alternatives are presented concerning the most frequently used combinations of buffer solutions applied for development and analysis of immune chromatography test-systems. The preferences are established in the field of characteristics of membranes used in composition of multi-membrane composite of immune chromatographic test-systems.
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Clin Chim Acta
March 2025
College of Pharmacy, Xinjiang Medical University, Urumqi 830054, China; Key Laboratory of High Incidence Disease Research in Xingjiang (Xinjiang Medical University), Ministry of Education, Urumqi 830054, China; Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830013, China; Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830054, China. Electronic address:
Background And Aims: Ketamine and morphine are among the most commonly abused drugs. Ketamine is classified as a new psychoactive substance, while morphine represents the opioid class. These two substances may exhibit synergistic or antagonistic effects in their pharmacological actions, and their combined abuse could lead to more severe health risks, such as respiratory depression.
View Article and Find Full Text PDFIran J Allergy Asthma Immunol
February 2025
Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Human cytomegalovirus glycoprotein B (gB) emerges as a viable candidate for eliciting neutralizing antibodies. This research specifically focused on exploring the immune reaction prompted by the nonglycosylated variant of the gB, with a comprehensive assessment of humoral immunity in mice. The gB coding sequence was optimized and expressed in pET-15b.
View Article and Find Full Text PDFJ Am Soc Mass Spectrom
March 2025
Immunity, Inflammation, and Disease Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, United States.
The analysis of small carboxyl-containing metabolites (CCMs), such as tricarboxylic acid (TCA) cycle intermediates, provides highly useful information about the metabolic state of cells. However, their detection using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) methods can face sensitivity and specificity challenges given their low ionization efficiency and the presence of isomers. Ion mobility spectrometry (IMS), such as trapped ion mobility spectrometry (TIMS), provides additional specificity, but further signal loss can occur during the mobility separation process.
View Article and Find Full Text PDFPharmaceuticals (Basel)
January 2025
State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
As a medicinal and food homologous substance, is renowned for its potential health benefits, including anti-tumor effects, immune system support, and anti-inflammatory properties. polypeptides have demonstrated significant biological activity, including the regulation of coagulation and lipid metabolism. However, the peptide composition of requires further clarification to facilitate quality control improvements and a deeper investigation into its pharmacological effects.
View Article and Find Full Text PDFMedicina (Kaunas)
February 2025
Department of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool L7 8XZ, UK.
: Acute gastroenteritis (AGE) is a major contributor to pediatric morbidity and mortality worldwide. There is a scarcity of data on AGE in North Lebanon, a region profoundly affected by the Syrian refugee crisis and water sanitation issues. This study examines the prevalence, risk factors, and seasonal variations in adenovirus and rotavirus infections in children with AGE in North Lebanon.
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