The whole-genome sequence (WGS) of KP3771 isolate was characterized. This strain was recovered from the urine sample of an 80-year-old man hospitalized in an intensive care unit of the University Hospital Tahar Sfar in Tunisia. WGS using a MiSeq platform was used. The assembled genome was subjected to several software analyses. KP3771 was resistant to all antibiotics but colistin and tigecycline. WGS analysis found 18 transmissible genes encoding resistance markers, including and genes, which were carried by four plasmids belonging to the Inc Ib, IIk, and R groups. Three families of genes encoding virulence factors were detected, including adhesins (, , , , , , and ), siderophores (enterobactin, aerobactin, and yersiniabactin siderophores), and protectin/invasin (). The strain was assigned to the sequence type 147. This study describes the genome of a carbapenemase-producing clinical isolate recovered in Tunisia. Bacteria WGS has become the reference technology to address epidemiological issues; this high level of information is particularly well suited to enrich epidemiological workflows' output.
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