AI Article Synopsis
- A rapid and precise diagnostic toolkit for detecting the H5N1 strain of avian influenza A has been developed, using quantum dots and a smartphone-based dual fluorescent system.
- This system employs two types of quantum dots to specifically target and enhance the detection of influenza A and its H5 subtype, allowing for simultaneous testing.
- The results exhibit high sensitivity (92.86% and 78.57%) and specificity (100% and 97.37%) for detecting these viruses, indicating that this method can significantly improve diagnosis for H5N1 infections.
Article Abstract
Accurate and rapid diagnosis of highly pathogenic avian influenza A H5N1 is of critical importance for the effective clinical management of patients. Here, we developed a rapid and simultaneous detection toolkit for influenza A H5 subtype viruses in human samples based on a bioconjugate of quantum dots (QDs) assembly and a smartphone-based rapid dual fluorescent diagnostic system (SRDFDS). Two types of QDs were assembled on a latex bead to enhance the detection sensitivity and specificity of influenza A infection (QD580) and H5 subtype (QD650). The dual signals of influenza A and H5 subtype of H5N1-infected patients were detected simultaneously and quantified separately by SRDFDS equipped with two emission filters. Our results showed a high sensitivity of 92.86% (13/14) and 78.57% (11/14), and a specificity of 100% (38/38, < 0.0001) and 97.37% (37/38) for influenza A and H5 subtype detection, respectively. Therefore, our multiplex QD bioconjugates and SRDFDS-based influenza virus detection toolkit potentially provide accurate and meaningful diagnosis information with improved detection accuracies and sensitivities for H5N1 patients.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299699 | PMC |
| http://dx.doi.org/10.7150/thno.28027 | DOI Listing |
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