AI Article Synopsis
- - The text discusses recent advancements in CRISPR/Cas9 genome editing technology, specifically its application to soybean and other plant species.
- - It outlines updated protocols for targeting single and multiple genes both in soybean and in different plant systems, including methods for delivering the necessary reagents.
- - Successful results have been achieved, with transgenic soybean plants created that have mutations in up to three targeted genes using the described techniques.
Article Abstract
CRISPR/Cas9 mediated genome editing technology has experienced rapid advances in recent years and has been applied to a wide variety of plant species, including soybean. Several platforms have been developed for designing and cloning of single CRISPR targets or multiple targets in a single destination vector. This chapter provides an updated working protocol for applying CRISPR/Cas9 technology to target a single gene or multiple genes simultaneously in soybean. We describe two platforms for cloning single CRISPR targets and multiplexing targets, respectively, and reagent delivery methodologies. The protocols address crucial limiting steps that can limit CRISPR editing in soybean hairy roots, composite plants, and tissue culture-based regenerated whole plants. To date, transgenic soybean plants with mutagenesis in up to three target genes have been obtained with this procedure.
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| http://dx.doi.org/10.1007/978-1-4939-8991-1_16 | DOI Listing |
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