AI Article Synopsis
- Subcellular localization is key to protein function, and this study offers a comprehensive analysis of protein localization across 12,418 genes in five cell lines using mass spectrometry.
- The research uncovers insights on how alternative splicing, protein domains, and growth factors influence protein localization, revealing that most proteins have a primary subcellular location and that splicing has minimal impact on this aspect.
- This work enhances our understanding of cellular architecture and is accessible to the public at www.subcellbarcode.org.
Article Abstract
Subcellular localization is a main determinant of protein function; however, a global view of cellular proteome organization remains relatively unexplored. We have developed a robust mass spectrometry-based analysis pipeline to generate a proteome-wide view of subcellular localization for proteins mapping to 12,418 individual genes across five cell lines. Based on more than 83,000 unique classifications and correlation profiling, we investigate the effect of alternative splicing and protein domains on localization, complex member co-localization, cell-type-specific localization, as well as protein relocalization after growth factor inhibition. Our analysis provides information about the cellular architecture and complexity of the spatial organization of the proteome; we show that the majority of proteins have a single main subcellular location, that alternative splicing rarely affects subcellular location, and that cell types are best distinguished by expression of proteins exposed to the surrounding environment. The resource is freely accessible via www.subcellbarcode.org.
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| http://dx.doi.org/10.1016/j.molcel.2018.11.035 | DOI Listing |
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