The site-directed mutagenesis of tomato allene oxide synthase LeAOS3 (CYP74C3) on the "F/L toggle" site resulted in a partial conversion of LeAOS3 into epoxyalcohol synthase.
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Galactinol Regulates JA Biosynthesis to Enhance Tomato Cold Tolerance.
J Agric Food Chem
February 2024
College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China.
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- Low temperatures negatively affect plant growth and fruit yield; this study shows that enhancing galactinol synthase expression can improve tomato cold tolerance.
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Plant Physiol
January 2024
National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, P.R. China.
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January 2023
Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, P.O. Box 261, 420111 Kazan, Russia.
The product specificity and mechanistic peculiarities of two allene oxide synthases, tomato LeAOS3 (CYP74C3) and maize ZmAOS (CYP74A19), were studied. Enzymes were vortexed with linoleic acid 9-hydroperoxide in a hexane-water biphasic system (20-60 s, 0 °C). Synthesized allene oxide (9,10-epoxy-10,12-octadecadienoic acid; 9,10-EOD) was trapped with ethanol.
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