The molecular and functional characterization of single cells at scale has emerged as a key driver to unravel tissue biology. Thus, it is important to understand the strengths and limitations of transcriptomic approaches, molecular barcoding and functional assays used to study cellular properties at the single-cell level. Here, we review recent relevant work from the haematopoietic system and discuss how to interpret and integrate data obtained with different technologies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/s41556-018-0227-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of clonal hematopoiesis and mosaic loss of Y chromosome in cardiovascular risk: An analysis in prospective studies.
Elife
December 2024
CHU de Bordeaux, Service des Maladies Coronaires et Vasculaires, Pessac, France.
Background: Clonal hematopoiesis of indeterminate potential (CHIP) was initially linked to a twofold increase in atherothrombotic events. However, recent investigations have revealed a more nuanced picture, suggesting that CHIP may confer only a modest rise in myocardial infarction (MI) risk. This observed lower risk might be influenced by yet unidentified factors that modulate the pathological effects of CHIP.
View Article and Find Full Text PDFTherapy-Related Myeloid Neoplasms: Complex Interactions among Cytotoxic Therapies, Genetic Factors, and Aberrant Microenvironment.
Blood Cancer Discov
November 2024
Department of Haematology, Royal Adelaide Hospital, Central Adelaide Local Health Network, Adelaide, Australia.
Article Synopsis
- Therapy-related myeloid neoplasm (t-MN) is a severe blood cancer linked to previous cytotoxic treatments and has a poor prognosis due to its aggressive behavior and treatment resistance.
- Recent research has shifted understanding from just genomic changes in blood stem cells to include interactions between genetic predispositions and the aging bone marrow environment.
- Recognizing risk factors for t-MN can help healthcare professionals better predict and potentially prevent its occurrence, leading to improved screening and treatment strategies for high-risk patients.
A new era of functional experimentation in human hematopoiesis and leukemia research.
Exp Hematol
December 2024
Department of Medicine, Division of Hematology, Cancer Institute, and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA.. Electronic address:
Article Synopsis
- Scientists have learned a lot about blood stem cells, including those involved in leukemia, through different experiments.
- Recently, new technologies have allowed researchers to gather more detailed information about human cells, but they're focusing more on describing things rather than testing how cells actually work.
- The authors believe that better tools for editing genes and studying stem cells will help scientists move from just describing cells to really understanding their functions more effectively.
The crosstalk between lung cancer and the bone marrow niche fuels emergency myelopoiesis.
Front Immunol
August 2024
Laboratory for Molecular and Cellular Therapy (LMCT), Translational Oncology Research Center (TORC), Department of Biomedical Sciences, Vrije Universiteit Brussel, Brussels, Belgium.
Modest response rates to immunotherapy observed in advanced lung cancer patients underscore the need to identify reliable biomarkers and targets, enhancing both treatment decision-making and efficacy. Factors such as PD-L1 expression, tumor mutation burden, and a 'hot' tumor microenvironment with heightened effector T cell infiltration have consistently been associated with positive responses. In contrast, the predictive role of the abundantly present tumor-infiltrating myeloid cell (TIMs) fraction remains somewhat uncertain, partly explained by their towering variety in terms of ontogeny, phenotype, location, and function.
View Article and Find Full Text PDFDNA damage repair in megakaryopoiesis: molecular and clinical aspects.
Expert Rev Hematol
October 2024
Thalassemia & Hemoglobinopathy Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Introduction: Endogenous DNA damage is a significant factor in the damage of hematopoietic cells. Megakaryopoiesis is one of the pathways of hematopoiesis that ends with the production of platelets and plays the most crucial role in hemostasis. Despite the presence of efficient DNA repair mechanisms, some endogenous lesions can lead to mutagenic alterations, disruption of pathways of hematopoiesis including megakaryopoiesis and potentially result in human diseases.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!