HPLC-DAD-SPE-NMR isolation of tetracyclic spiro-alkaloids with antiplasmodial activity from the seeds of .

Seven tetracyclic spiro-alkaloids, i.e. glucoerysodine (), erysodine (), -erythratidine (), erysovine (), erythratidine (), erysotrine () and erythraline () were isolated from the seeds of by means of conventional separation methods and HPLC-DAD-SPE-NMR. Their structures were elucidated by spectroscopic means. This is the first report on the isolation of compounds , and from this plant. Antiplasmodial activity against the chloroquine-resistant strain K1 and cytotoxicity against MRC-5 cells (human fetal lung fibroblast cells) was assessed . Erysodine () and erysovine () showed moderate activity (IC 6.53 µM and 4.05 µM, respectively), compared with the standard chloroquine (IC = 0.14 µM). No cytotoxicity was observed in a concentration up to 64.0 µM.