A Dual Selection Marker Transformation System Using for the Industrial 3.042.

Currently, the genetic modification of is mainly dependent on protoplastmediated transformation (PMT). In this study, we established a dual selection marker system in an industrial 3.042 strain by using -mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of 3.042.