In teleosts, sex is plastic and is influenced by environmental factors. Elevated temperatures have masculinizing effects on the phenotypic sex of certain sensitive species. In this study, we reared genetic XX Japanese flounder at a high temperature (27.5 ± 0.5 °C) and obtained a population of sex-reversal XX males (male ratio, 95.24%). We comparatively analyzed the dynamic characteristics of germ cells and gsdf (gonadal soma-derived factor) expression during sexual differentiation for the experimental (27.5 ± 0.5 °C) and control (18 °C ± 0.5 °C) groups. The results revealed that the germ cell proliferation inhibited and gsdf expression up-regulated in the experimental group, and the gsdf mRNA and proteins expressed in somatic cells that had direct contact with germline stem cells (with Nanos 2 protein expression) including spermatogonia and oogonia by ISH (in situ hybridization) and IHC (immunohistochemistry). In addition, we also overexpressed the gsdf in XX flounders, and the germ cell number of XX flounders bearing gsdf gene significantly decreased and sometimes disappeared completely, which was consistent with the results from high-temperature induction. Therefore, based on all the results, we speculated that the high expression of gsdf might inhibit germ cell proliferation during sex differentiation, and eventually cause sex reversal in the high-temperature induced masculinization of XX Japanese flounder.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ygcen.2018.12.012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development and Application of PSCPL13 Probiotics in Olive Flounder () Farming.
Microorganisms
January 2025
Laboratory of Veterinary Pharmacokinetics, Institute for Veterinary Biomedical Science, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
Aquaculture has grown significantly, contributing to global food security and sustainability; however, intensified fish farming has increased disease susceptibility and antibiotic resistance. This study assessed the probiotic potential of PSCPL13 (hereafter, PSCPL13), isolated from the intestines of Japanese eels, for enhancing the health of olive flounder. After screening 16 isolates, PSCPL13 was selected because of its potential broad-spectrum antibacterial activity against many pathogens, such as and .
View Article and Find Full Text PDFtgfβ1a/vegfa gene expression and methylation in response to acute hypoxia in Japanese flounder (Paralichthys olivaceus).
Dev Comp Immunol
January 2025
Key Laboratory of Mariculture, Ocean University of China, Ministry of Education, Qingdao, 266003, PR China. Electronic address:
The physiological response and molecular mechanism of the immune response of Japanese flounder under hypoxia stress remain unclear. In this study, we examined the immune-related physiological indexes and the molecular mechanisms of Japanese flounders under acute hypoxia stress. The results showed that the levels of serum ALT, ALP, AST and LDH in hypoxia stress group were significantly increased (P < 0.
View Article and Find Full Text PDFEffects of nitrate (NO) stress-induced exacerbated cadmium (Cd) toxicity on the inflammatory response, oxidative defense, and apoptosis in juvenile Japanese flounder (Paralichthys olivaceus).
J Environ Sci (China)
June 2025
Laboratory of Pathology and Immunology of Aquatic Animals/Jiangsu Key Laboratory of Marine Biotechnology, School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Lianyungang 222005, China. Electronic address:
Due to the discharge of industrial wastewater, urban domestic sewage, and intensive marine aquaculture tailwater, nitrate (NO) pollution has emerged as a significant issue in offshore waters. Nitrate pollution affects aquatic life and may interact with other pollutants, leading to comprehensive toxicity. Cadmium (Cd) is the most widespread metal contaminant, adversely affecting aquatic life in the coastal waters of China.
View Article and Find Full Text PDFSynchronously Mature Intersex Japanese Flounder (): A Rare Case.
Animals (Basel)
October 2024
China State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Beidaihe Central Experiment Station, Chinese Academy of Fishery Sciences, Qinhuangdao 066100, China.
Japanese flounder is usually gonochoristic, with gonads that are either testes or ovaries. Here, we report an unusual case of hermaphroditism in Japanese flounder captured from the Bohai Sea. In the intersex flounder, the membrane of the upper ovary was closely connected to the abdominal muscles and internal organs, and the eggs filled the entire abdomen.
View Article and Find Full Text PDFIdentification, expression, and function analysis of Rbpms2 splicing variants in Japanese flounder gonad.
Gen Comp Endocrinol
December 2024
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China. Electronic address:
Rbpms2, an RNA-binding protein with multiple splicing (Rbpms), can interact with RNAs to involve oocyte development, thereby influencing female sex differentiation in vertebrates. Here, two splicing variants of the Rbpms2 gene from Japanese flounder (Paralichthys olivaceus) were identified, namely Rbpms2.1 and Rbpms2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!