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poreTally: run and publish de novo nanopore assembler benchmarks. | LitMetric

poreTally: run and publish de novo nanopore assembler benchmarks.

Bioinformatics

Bioinformatics Group, WUR, 6700 AP Wageningen, The Netherlands.

Published: August 2019

Summary: Nanopore sequencing is a novel development in nucleic acid analysis. As such, nanopore-sequencing hardware and software are updated frequently and extensively, which quickly renders peer-reviewed publications on analysis pipeline benchmarking efforts outdated. To provide the user community with a faster, more flexible alternative to peer-reviewed benchmark papers for de novo assembly tool performance we constructed poreTally, a comprehensive benchmarking tool. poreTally automatically assembles a given read set using several often-used assembly pipelines, analyzes the resulting assemblies for correctness and continuity, and finally generates a quality report, which can immediately be published on Github/Gitlab.

Availability And Implementation: poreTally is available on Github at https://github.com/ cvdelannoy/poreTally, under an MIT license.

Supplementary Information: Supplementary data are available at Bioinformatics online.

Download full-text PDF

Source
http://dx.doi.org/10.1093/bioinformatics/bty1045DOI Listing

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