AI Article Synopsis
- The study explores a new in vitro model that integrates human muscle fibers, fibroblasts, and a vascular network to mimic the complex interactions found in skeletal muscle.
- It highlights how this model can induce muscle-specific blood vessel formation and allows fibroblasts to self-organize, which is crucial for understanding muscle responses in health and disease.
- This advanced system aims to better replicate the conditions of muscular dystrophies and offers insights into fibrosis that traditional 2D cell cultures fail to provide.
Article Abstract
The integration of vascular structures into in vitro cultured tissues provides realistic models of complex tissue-vascular interactions. Despite the incidence and impact of muscle-wasting disorders, advanced in vitro systems are still far from recapitulating the environmental complexity of skeletal muscle. Our model comprises differentiated human muscle fibers enveloped by a sheath of human muscle-derived fibroblasts and supported by a vascular network with mural-like cells. Here, we demonstrate the induction of muscle-specific endothelium and the self-organization of endomysial muscle fibroblasts mediated by endothelial cells. We use this model to mimic the fibrotic environment characterizing muscular dystrophies and to highlight key signatures of fibrosis that are neglected or underestimated in traditional 2D monocultures. Overall, this vascularized meso-scale cellular construct finely recapitulates the human skeletal muscle environment and provides an advanced solution for in vitro studies of muscle physiology and pathology.
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| http://dx.doi.org/10.1016/j.celrep.2018.11.092 | DOI Listing |
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