Selenite enrichment broth (SEB) is used to optimize the recovery of Salmonella enterica subspecies enterica from stool samples. Compared to a direct culture approach, it enhances culture yield by reducing growth of faecal coliforms and faecal streptococci. Over the course of seven years from 2000 to 2017, 47,235 faecal samples were tested with a Salmonella PCR. We investigated the added value of using SEB in combination with faeces for DNA extraction, in order to improve the sensitivity of molecular diagnostics for detection of Salmonella. A Salmonella enterica subspecies enterica strain was tested for growth characteristics, with and without incubation in SEB, to determine the impact of Selenite enrichment in the Salmonella PCR. Retrospectively, a total of 102 Salmonella enterica subspecies enterica PCR positive faecal samples were re-analysed. DNA extraction was performed with the EasyMag® and MagNaPure96® system using three different input volumes of faeces and SEB. Prospectively, 114 Salmonella PCR positive faecal samples were retested within 2 days using five different input volumes for DNA extraction. Retrospectively, PCR that used SEB as part of input in the DNA extraction, 7/102 (7%) Salmonella PCR positive samples were additionally detected compared to no use of SEB. Of these, Salmonella enterica subspecies enterica serovariation Thompson, Enteritidis, 9,12:l.v and Senftenberg have been outbreak related in the past. Prospectively results were combined in collaboration with another microbiology laboratory, 15/114 (13.2%) additional specimens were detected with the Salmonella PCR, including processing Selenite enrichment broth. In conclusion, of the total 47,235 feacal samples, with SEB the prevalence of a positive PCR for Salmonella is 2.2%. Of these 2.2% positive Salmonella PCRs, 0.4% was not detected in culture. By using SEB an improved detection of Salmonella diagnostics could be realized and a substantial part of 13,2% additional Salmonella cases could be detected.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.mimet.2018.12.018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sodium selenite enhanced the selenium content in black soldier fly.
Sci Rep
January 2025
College of Animal Science, Anhui Science and Technology University, Bengbu, 233100, People's Republic of China.
This study focuses on the effects of different levels of sodium selenite on the growth, selenium content, and antioxidant capacity of black soldier fly (Hermetia illucens). The experiment used different doses of sodium selenite for treatment, including a basic diet with no supplements (control) and diets supplemented with 10 mg/kg (Se10), 20 mg/kg (Se20), 30 mg/kg (Se30), and 40 mg/kg (Se40) sodium selenite, and results show that sodium selenite supplementation significantly increases selenium content and improves selenium utilization and antioxidant capacity (P < 0.05).
View Article and Find Full Text PDFEffects of exogenous selenium application on quality characteristics, selenium speciation, and in vitro bioaccessibility of rice pancakes.
Food Chem X
January 2025
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Key Laboratory of Industrial Microbiology, School of Life and Health Sciences, Hubei University of Technology, Wuhan, Hubei 430068, PR China.
Selenium is an essential trace element for human health. To date, a hotspot of functional foods is strengthening the content of organic Se in food using biological Se enrichment. Herein, Se-enriched rice pancakes were produced by directly adding different sodium selenite concentrations into the fermentation process.
View Article and Find Full Text PDFCovalent Modification of Selenium in Polysaccharide Enhances Immunoregulation Activity via the TLR4-Mediated MAPK/NF-κB Signaling Pathway.
J Agric Food Chem
December 2024
College of Life Science, Northwest Normal University, Lanzhou 730070, People's Republic of China.
Selenium (Se) is a crucial trace element that demonstrates significant immunomodulatory effects, which are attributed to the variability in its valence states and metabolic pathways. To investigate the Se-related immunoregulatory effects, locust bean gum (LBG), a typical galactomannan, was selenized by employing deep eutectic solvents (DESs) as high-efficiency solvents to obtain Se-covalent modified LBG (SeLBGs) with similar molecular mass and different Se contents (SeLBG, 1049.57 and SeLBG, 4926.
View Article and Find Full Text PDFCharacterization of the effect of low-concentration sodium selenite on the microstructure and quality of yeast-leavened steamed bread using X-ray computed tomography.
Food Chem
December 2024
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Key Laboratory of Industrial Microbiology, School of life and health sciences, Hubei University of Technology, Wuhan, Hubei 430068, PR China.
Dough fermentation is an effective method for selenium conversion. This study investigated the effects of low NaSeO concentrations on the morphology, texture, fermentation properties, Se species, Se bioaccessibility, and antioxidant capacity of two types of yeast-leaved steamed bread. The results indicated that NaSeO did not significantly affect the specific volume; but it did result in increased hardness.
View Article and Find Full Text PDFEffects of straw amendment on the bioavailability of selenite in soil and its mechanisms.
Ecotoxicol Environ Saf
December 2024
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi 712100, China. Electronic address:
Dissolved organic matter (DOM) released by straw returning for decomposition interacts with selenium (Se) in soil, which affects the speciation distribution of Se and its bioavailability. However, the relative mechanisms involved are slightly understood. This study investigated the effects of straw-derived DOM on two levels of exogenous selenite (low-Se and high-Se treatments) in two types of soil with distinct pH.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!