Many-year experience of using authors' own systems for steroid radioimmunoassay (RIA) is summarized. Synthesis of natural and anabolic steroid derivatives and their conjugation with bovine serum albumin are considered. Schemes of rabbits immunization with steroid-protein conjugates, and the antisera characteristics are described. Data on comparison of 3H- and 125I-ligands in steroid RIA are presented as well as the characteristics of the RIA systems. The RIA optimization and quality control problems as well as the prospects of the RIA development are discussed.
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