Cyclooxygenases are responsible for the production of prostaglandin H (PGH) from arachidonic acid. PGH can be converted into some bioactive prostaglandins, including prostaglandin F (PGF), a potent chemical messenger used as a biological regulator in the fields of obstetrics and gynecology. The chemical messenger PGF has been industrially produced by chemical synthesis. To develop a biotechnological process, in which PGF can be produced by a microorganism, we transformed an oleaginous fungus, Mortierella alpina 1S-4, rich in triacylglycerol consisting of arachidonic acid using a cyclooxygenase gene from a red alga, Gracilaria vermiculophylla. PGF was accumulated not only in the mycelia of the transformants but also in the extracellular medium. After 12 days of cultivation approximately 860 ng/g and 6421 µg/L of PGF were accumulated in mycelia and the extracellular medium, respectively. The results could facilitate the development of novel fermentative methods for the production of prostanoids using an oleaginous fungus.
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