Chemical characterization of constituents from Hua and their cytotoxic and antioxidant evaluation.

Twenty-four compounds were isolated from the roots of Hua, including a new octopamine dimer, named -bis(-feruloyl)octopamine . The structure was established on the basis of spectroscopic and chemical methods. All the extracts and compounds were evaluated for cytotoxic and antioxidant activities by using MTT and chemiluminescence assay. The extracts showed activity against MCF-7 and HepG-2 cell lines from IC 0.30 to 1.01 mg mL. Compound exhibited activity against HepG-2 cell lines with IC 8.99 μM. Compound exhibited activity against Hela cell lines with IC 2.53 μM and BGC-823 cell lines with IC 7.77 μM. Moreover, compound showed antioxidant with IC 12 µM compared to the positive control with IC 77 µM. Compound exhibited activity against HepG-2 cell lines with IC 1.05 μM and MCF-7 cell lines with IC 1.89 μM. These results indicated that this plant might be potential in natural medicine and healthy food.