Identification of an intermediate for 1,8-dihydroxynaphthalene-melanin synthesis in a race-2 isolate of (syn. ).

Heliyon

Cátedra de Microbiología Agrícola, Departamento de Ciencias Biológicas, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 119, 1900 La Plata, Argentina.

Published: December 2018

(syn. , Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by and to elucidate its biochemical pathway. We isolated a black pigment from cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6299106PMC
http://dx.doi.org/10.1016/j.heliyon.2018.e01036DOI Listing

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