(syn. , Mycosphaerellaceae) is a dematiaceous fungus that causes tomato leaf mould. It is characterized by its biotrophic lifestyle and the synthesis of the bianthraquinone secondary metabolite, cladofulvin. The aim of the study was to characterize the dark pigment photochemically synthesized by and to elucidate its biochemical pathway. We isolated a black pigment from cultures of the fungus. We determined the pigment to be 1,8-dihydroxynaphthalene (DHN)-melanin based on its chemical and photochemical characteristics, as well as the presence of flaviolin, when fungal reductases were inhibited by tricyclazole. Furthermore, the gene involved in pigment synthesis has a KS domain already associated with DHN-melanin. Our findings support the relevance of studying melanization in .
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http://dx.doi.org/10.1016/j.heliyon.2018.e01036 | DOI Listing |
Sci Rep
December 2024
Institute for Forest Resources and Environment of Guizhou, College of Forestry, Guizhou University, Guiyang, 550025, Guizhou, China.
This study aims to explore the low phosphorus (P) tolerance of saplings from different Gleditsia sinensis Lam. families. It also seeks to screen for Gleditsia sinensis families with strong low P tolerance and identify key indicators for evaluating their tolerance.
View Article and Find Full Text PDFSci Rep
December 2024
Department of Computer Science and Digital Technologies, University of East London, London, UK.
Nursing activity recognition has immense importance in the development of smart healthcare management and is an extremely challenging area of research in human activity recognition. The main reasons are an extreme class-imbalance problem and intra-class variability depending on both the subject and the recipient. In this paper, we apply a unique two-step feature extraction, coupled with an intermediate feature 'Angle' and a new feature called mean min max sum to render the features robust against intra-class variation.
View Article and Find Full Text PDFJ Antimicrob Chemother
December 2024
Food and Veterinary Institute Oldenburg, Lower Saxony State Office for Consumer Protection and Food Safety (LAVES), Oldenburg, Germany.
Antimicrobial susceptibility testing (AST) in the veterinary sector by broth microdilution is mainly based on commercially available microtitre plates with specific panels. A critical review of commercially available microtitre panels identified AST panels that fulfil the requirements for obtaining reliable AST results by covering the necessary antimicrobial concentrations for both clinical breakpoints as well as quality control (QC) ranges for approved QC strains. However, there are AST panels in which these prerequisites are only in part fulfilled, and some AST panels that do not fulfil the aforementioned criteria at all.
View Article and Find Full Text PDFOpen Vet J
November 2024
Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Wasit, Wasit, Iraq.
Background: In goats, acute and chronic respiratory infections are often characterized by a rapidly progressing clinical course with little opportunity to develop an effective antibiotic therapy.
Aim: This study aimed to identify spp. in pneumonic goats, assess its antibiotic susceptibility, and confirm the molecular phylogenetics of spp.
Parasit Vectors
December 2024
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis and College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
Background: Echinococcosis is a zoonotic disease caused by an Echinococcus tapeworm infection. While diagnostic methods for humans often rely on ultrasound imaging and immunodiagnostic techniques, diagnosis in intermediate hosts typically has no widely used diagnostic markers, hampering disease control efforts.
Methods: The differences in serum metabolites of sheep infected with Echinococcus granulosus and a control group were analyzed using ultrahigh-performance liquid chromatography (UHPLC) separation with tandem mass spectrometry (MS/MS) detection.
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