A method was developed for the simultaneous determination of anserine, homocarnosine and carnosine in meat samples using ion chromatography (IC) with integrated pulsed amperometric detection (IPAD). The samples were separated on a high performance anion exchange AminoPac PA10 column (250 mm×2 mm) using 100 mmol/L NaOH as the eluent. The flow rate was kept at 0.2 mL/min and the column temperature was set at 30℃. The three target compounds were separated within 15 min, and there were no interferences from 17 tested amino acids in their determination. Under the optimal chromatographic conditions, anserine, homocarnosine and carnosine showed good linearity in the range of 0.05-5 mg/L with correlation coefficients () greater than 0.99. The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 8.9-22.1 μg/L and 29.6-73.6 μg/L, respectively. The proposed method was applied to the analysis of duck breast and goose breast meat samples. The average spiked recoveries ranged from 92.4% to 104.5%. This simple and sensitive method can be applied to the determination of related nutrients in meat products.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3724/SP.J.1123.2018.08029 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Endogenous histidine peptides are physiological antioxidants that prevent oligodendrocyte cell death and myelin loss in vivo.
Glia
January 2025
Department of Biochemistry. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Histidine dipeptides (HDs) are synthesized in brain oligodendrocytes by carnosine synthase (carns1), but their role is unknown. Using metabolomics and in vivo experiments with both constitutive and oligodendrocyte-selective carns1-KO mouse models, we found that HDs are critical for oligodendrocyte survival and protect against oxidative stress. Carns1-KO mouse models had lower numbers of mature oligodendrocytes, increased lipid peroxidation, and behavioral changes.
View Article and Find Full Text PDFMetabolome and Transcriptome Profiling Reveals Age-Associated Variations in Meat Quality and Molecular Mechanisms of Taihe Black-Bone Silky Fowls.
J Agric Food Chem
October 2024
College of Animal Science, Zhejiang University, Hangzhou 310030, China.
To explore the changes in meat quality and molecular mechanisms during the growth and development of Taihe black-bone silky fowl, this study employed liquid chromatography-mass spectrometry (LC-MS/MS) metabolomics to elucidate the dynamic changes of key differential metabolites (DMs) affecting meat quality, indicating that chicken at D120 had higher levels of ω-3 polyunsaturated fatty acids (PUFAs), creatine, anserine, and homocarnosine, while D150 had the most stachydrine and D210 had the most acylcarnitines. Additionally, D120 and D180 had more umami and sweet compounds. Furthermore, key metabolic pathways influenced by age included purine metabolism, the pentose phosphate pathway, nicotinate and nicotinamide metabolism, and taurine and hypotaurine metabolism.
View Article and Find Full Text PDFHistidine-containing dipeptide deficiency links to hyperactivity and depression-like behaviors in old female mice.
Biochem Biophys Res Commun
October 2024
Program of Food and AgriLife Science, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, 739-8527, Japan; Smart Agriculture, Graduate School of Innovation and Practice for Smart Society, Hiroshima University, Hiroshima, 739-8527, Japan. Electronic address:
Article Synopsis
- Carnosine, anserine, and homocarnosine are important dipeptides found in muscles and the brain, with carnosine and anserine extensively studied for their effects on muscle function and exercise performance.
- Research on homocarnosine, primarily in the brain, is limited, leading to an investigation of its role in behaviors using carnosine synthase-1-deficient mice.
- The study observed that older Carns1 mice displayed hyperactivity and signs of depression along with altered hormone levels, while homocarnosine deficiency had subtle effects on GABA levels in the brain, suggesting that these dipeptides may play a significant role in behavior and potential neurological disorders.
Binding Modes of Carnostatine, Homocarnosine, and Ophidine to Human Carnosinase 1.
ACS Omega
November 2023
Department of Chemistry, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Carnosine (CAR), anserine (ANS), homocarnosine (H-CAR), and ophidine (OPH) are histidine-containing dipeptides that show a wide range of therapeutic properties. With their potential physiological effects, these bioactive dipeptides are considered as bioactive food components. However, such dipeptides display low stability due to their rapid degradation by human serum carnosinase 1 (CN1).
View Article and Find Full Text PDFMetabolomic profiling of amino acids study reveals a distinct diagnostic model for diabetic kidney disease.
Amino Acids
November 2023
Research Institute of Nephrology, Zhengzhou University, Zhengzhou, 450000, China.
Diabetic kidney disease (DKD), a highly prevalent complication of diabetes mellitus, is a major cause of mortality in patients. However, identifying circulatory markers to diagnose DKD requires a thorough understanding of the metabolic mechanisms of DKD. In this study, we performed ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) to reveal altered metabolic profiles of amino acids (AAs) in patients with DKD.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!