Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform.

Chem Commun (Camb)

Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.

Published: January 2019

We demonstrate the synthesis of protein-polymer hybrid hydrogel that can be used as a platform for immobilizing functional proteins. Orthogonal chemistry was employed for cross-linking the hybrid network and conjugating proteins to the gel backbone, allowing for the convenient, one-pot formation of a functionalized hydrogel. The resulting hydrogel had tunable mechanical properties, was stable in solution, and biocompatible.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370476PMC
http://dx.doi.org/10.1039/c8cc08720bDOI Listing

Publication Analysis

Top Keywords

protein-polymer hybrid
8
hybrid hydrogel
8
engineering bioorthogonal
4
bioorthogonal protein-polymer
4
hydrogel
4
hydrogel functional
4
functional protein
4
protein immobilization
4
immobilization platform
4
platform demonstrate
4

Similar Publications

Self-assembled nanoparticles of hybrid elastin-like and Oncostatin M polymers for improved wound healing.

Biomater Adv

December 2024

CBMA (Centre of Molecular and Environmental Biology)/ Aquatic Research Network (ARNET) Associate Laboratory, Department of Biology, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal; IB-S Institute of Science and Innovation for Sustainability, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal. Electronic address:

Oncostatin M (OSM) is a pleiotropic cytokine that can significantly enhance wound healing. Here, we report on the use of nanoparticles (NPs) formulated from a genetically engineered A200_hOSM protein polymer, which combines an elastin-like recombinamer (A200) with human OSM (hOSM) in the same molecule, aiming at enhancing wound healing processes. A200_hOSM NPs were obtained by self-assembly and evaluated for their bioactivity in human keratinocytes and fibroblasts.

View Article and Find Full Text PDF

Engineering RAFT Polymers to the Protein-capped Gold Nanoclusters for Developing Fluorescent Polymeric Nanoconjugates.

Macromol Biosci

December 2024

Department of Chemistry, École Centrale School of Engineering, Mahindra University, Hyderabad, 500043, India.

The synthesis of fluorescent hybrid nanomaterials engineered via the chain-end modification of reversible addition-fragmentation chain-transfer (RAFT) polymers on the surface of bovine serum albumin (BSA) protein-stabilized gold nanoclusters (AuNCs@BSA) is described. Based on the "grafting-to" approach the core-shell structured nanoconjugates AuNCs@BSA/polymer are generated via effective ligation of hydrophilic, and stimuli-responsive polymers. Such nanomaterials are characterized via various microscopic and spectroscopic studies and exhibit their size as ≈5 nm and emission peak at ≈650 nm.

View Article and Find Full Text PDF

Hypothesis: While albumin-coated microbubbles are routine contrast agents for ultrasound imaging, their short duration of contrast enhancement limits their use, yet can be improved by incorporating protein-copolymer hybrids into microbubble shells. The incorporation of N-vinyl-2-pyrrolidone and acrylic acid copolymer (P(VP-AA)) has been shown to enhance the performance of bovine serum albumin (BSA)-coated microbubbles. However, the impact of the copolymer structural properties on key microbubble characteristics (i.

View Article and Find Full Text PDF

Artificial Zymogen Based on Protein-Polymer Hybrids.

Biomacromolecules

November 2024

Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, United States.

This study explores the synthesis and application of artificial zymogens using protein-polymer hybrids to mimic the controlled enzyme activation observed in natural zymogens. Pro-trypsin (pro-TR) and pro-chymotrypsin (pro-CT) hybrids were engineered by modifying the surfaces of trypsin (TR) and chymotrypsin (CT) with cleavable peptide inhibitors utilizing surface-initiated atom transfer radical polymerization. These hybrids exhibited 70 and 90% reductions in catalytic efficiency for pro-TR and pro-CT, respectively, due to the inhibitory effect of the grafted peptide inhibitors.

View Article and Find Full Text PDF

Despite the substantial advancement in developing various hydrogel microparticle (HMP) synthesis methods, emulsification through porous medium to synthesize functional hybrid protein-polymer HMPs has yet to be addressed. Here, the aided porous medium emulsification for hydrogel microparticle synthesis (APME-HMS) system, an innovative approach drawing inspiration from porous medium emulsification is introduced. This method capitalizes on emulsifying immiscible phases within a 3D porous structure for optimal HMP production.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!