Background: Involvement of microRNAs in tumor development and their potential as prognostic biomarkers had been well acknowledged. However, the expression, clinical significance, and functional mechanisms of microRNA (miR)-302a-3p in hepatocellular carcinoma (HCC) have not been reported.
Patients And Methods: Real-time quantitative polymerase chain reaction was used to evaluate the expression of miR-302a-3p in 111 HCC tissues and adjacent normal liver tissues. Its association with clinicopathological characteristics was analyzed by the chi-square test. The Kaplan-Meier univariate survival analysis and multivariate Cox regression analysis were used to identify the clinical significance of miR-302a-3p in the overall survival (OS) of HCC patients. Transfection of miR-302a-3p mimics into HepG2 and Huh7 HCC cell lines was conducted to reveal its underlying mechanism in regulating HCC progression.
Results: miR-302a-3p expression was significantly decreased in HCC tissues compared with that in paired adjacent normal liver tissues (=0.005). miR-302a-3p expression was correlated with tumor number (=0.003), tumor size (<0.001), and tumor TNM stage (=0.028). The Kaplan-Meier survival analysis showed that patients in the high miR-302a-3p expression group had a better OS than those in the low miR-302a-3p expression group (=0.002). Multivariate analysis confirmed that miR-302a-3p expression can be used as an independent predictor for HCC prognosis (HR=0.480, 95% CI=0.249-0.894, =0.039). Proliferation, migration, and invasion capacities were all decreased in cells transfected with miR-302a-3p mimics. Moreover, our data showed a direct effect of miR302a-3p on inhibiting the expression and signaling of PRKACB in HCC cells.
Conclusions: miR-302a-3p serves as a tumor suppressor in HCC progression by directly inhibiting tumor proliferation and invasion, and its low expression is a potential biomarker for predicting a poor prognosis of HCC patients.
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| http://dx.doi.org/10.2147/OTT.S167162 | DOI Listing |
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