Multi-agent simulation regulated by microbe-oriented thermodynamics and kinetics equations for exploiting interspecies dynamics and evolution between methanogenesis, sulfidogenesis, hydrogenesis and exoelectrogenesis.

Multi-agent simulation (MAS) regulated by microbe-oriented thermodynamics and kinetics equations were performed for exploiting the interspecies dynamics and evolution in anaerobic respiration and bioelectrochemical systems. A newly-defined kinetically thermodynamic parameter is recognized microbes as agents in various conditions, including electron donors and acceptors, temperature, pH, etc. For verification of the MAS, the treatment of synthetic wastewater containing glucose and acetate was evaluated in four 25°C laboratory-scale reactors with different electron acceptors and cathode materials that had potential for methanogenesis, hydrogenesis, sulfidogenesis and exoelectrogenesis. Within 1000 h operation, the reactors performance and microbial structures using 16S rRNA sequencing matched with the MAS, suggesting acetoclastic exoelectrogenesis predominance (Geobacter). After 2400 h, MAS observed the co-existence of acetoclastic methanogenesis and acetoclastic and propionate exoelectrogenesis, as was reported previously. Such microbial evolution from the short-term to long-term operation likely resulted from the glucose-driven propionate. The MAS developed is applicable in a wide range of complex engineering and natural ecosystems.