Description of Wenzhouxiangella salilacus sp. nov., a moderate halophilic bacterium isolated from a salt lake in Xinjiang Province, China.

A Gram-stain negative, non-motile, strictly aerobic and rod-shaped bacterium, designated as 15181, was isolated from a salt lake in Xinjiang Province, China. Strain 15181 was able to grow at 10-40 °C (optimum 37 °C), pH 6.0-8.5 (optimum 7.0) and with 1-14% NaCl (optimum 4%, w/v). According to phylogenetic analysis based on 16S rRNA gene sequences, strain 15181 was assigned to the genus Wenzhouxiangella with high 16S rRNA gene sequence similarity of 97.4% to Wenzhouxiangella sediminis XDB06, followed by Wenzhouxiangella marina KCTC 42284 (95.9%). Strain 15181 exhibited ANI values of 80.0% and 72.0% to W. sediminis XDB06 and W. marina KCTC 42284, respectively. The in silico DDH analysis revealed that strain 15181 shared 19.1% and 18.7% DNA relatedness with W. sediminis XDB06 and W. marina KCTC 42284, respectively. Chemotaxonomic analysis showed that the sole respiratory quinone was ubiquinone-8, the major fatty acids included iso-C, iso-C and summed feature 9 (C 10-methyl and/or iso-Cω9c). The major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified glycolipids, two unidentified phospholipids, two unidentified aminophospholipids and an unidentified lipid. On the basis of phenotypic, genotypic and chemotaxonomic characteristics presented in this study, strain 15181 is concluded to represent a novel species in the genus Wenzhouxiangella, for which the name Wenzhouxiangella salilacus sp. nov. is proposed. The type strain is 15181 (=KCTC 62172=MCCC 1K03442).