Genotyping of isolates in healing process of bovine clinical mastitis.

In the present work, macrorestriction analysis was applied to characterize 44 field strains isolated from lactating cows suffering from mastitis in three dairy herds in Hesse State, Germany. Analysis of the obtained data by Pulse-Field Gel Electrophoresis (PFGE) showed that most of the isolates originating from different herds and cows were not related to each other. However, identical macrorestriction patterns were noted in 12 of 13 mastitic quarters in healing process, in three quarters even over the whole sampling period indicating persistent infection. In the present work, PFGE could detect variable levels of similarity ranging from 76 to 100%. The macrorestriction analyses revealed the presence of 10 PFGE pattern with more than four bands difference. PFGE profiles with minor differences (only one to three bands) were considered to be subtypes. The use of sensitive genotyping methods like macrorestriction analyses by PFGE enables the differentiation among new and persistent infections. Nevertheless minor changes in macrorestriction profiles could occur which are clearly distinguishable from totally unrelated strains.