A sensitive, microplate assay is described for the detection of a wide range of proteolytic enzymes, using radio-iodine-labeled gelatin as substrate. The technique uses the Bolton-Hunter reagent to label the substrate, which is then coated onto the wells of polyvinyl chloride microtiter plates. By measuring the radioactivity released the assay is able to detect elastase, trypsin, and collagenase in concentrations of 1 ng/ml or less, while the microtiter format permits multiple sample handling and minimizes sample volumes required for analysis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0003-2697(88)90444-7 | DOI Listing |
Publication Analysis
Top Keywords
sensitive microplate
8
microplate assay
8
proteolytic enzymes
8
assay detection
4
detection proteolytic
4
enzymes radiolabeled
4
radiolabeled gelatin
4
gelatin sensitive
4
assay described
4
described detection
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!