Characterization of a novel lytic myophage, phiA8-29, infecting Aeromonas strains.

Arch Virol

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.

Published: March 2019

AI Article Synopsis

  • Researchers isolated a new phage called Aeromonas virus phiA8-29 from the digestive tract of a freshwater fish, known for targeting harmful bacteria.
  • This phage has a long contractile tail, classifying it under the Myoviridae family, and its genome consists of double-stranded DNA with about 185 genes.
  • Genetic analysis shows that phiA8-29 has unique characteristics, making it a novel lytic Myoviridae phage that doesn't fit into any previously identified genera.

Article Abstract

Members of the bacterial genus Aeromonas are important aquatic pathogens that cause severe fish diseases. Here, we characterize a novel lytic phage, Aeromonas virus phiA8-29, isolated from the alimentary tract of a freshwater fish. Transmission electron microscopy showed that phiA8-29 has a long contractile tail and thus can be classified as a member of the family Myoviridae. The phage genome was identified as a double-stranded DNA molecule of 144,974 bp containing 185 open reading frames and nine tRNA-encoding genes. Comparative genomic analysis revealed that the phiA8-29 genome has little similarity to any of the currently sequenced Aeromonas phage genomes. Our data indicate that phiA8-29 is a novel lytic Myoviridae phage that does not belong to any of the known genera.

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Source
http://dx.doi.org/10.1007/s00705-018-4109-yDOI Listing

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