AI Article Synopsis
- Researchers transformed NIH3T3 cells using 3-methylcholanthrene (3-MCA) and phorbol-12-myristate-13-acetate (PMA) to study tumorigenic mechanisms at the transcriptional level.
- They assessed transformation through Giemsa staining and a methylcellulose colony formation assay, identifying changes in gene expression via a Mouse Genome 430 2.0 microarray.
- Five signaling pathways were linked to the transformation process, suggesting key roles for specific oncogenes and a tumor suppressor gene in these pathways.
Article Abstract
To analyse the mechanism of tumourigenic transformation of NIH3T3 cells at the transcriptional level, we used cancerogen 3-methylcholanthrene (3-MCA) and cancerogenic substance phorbol-12-myristate-13-acetate (PMA) to transform NIH3T3 cells and the assessment of transformation was performed using Giemsa staining and methylcellulose colony formation assay. Changes in gene expression profile were detected by Mouse Genome 430 2.0 microarray; and quantitative real-time polymerase chain reaction and Western blotting were used to verify the expression changes of mRNAs and proteins, respectively. With the aid of bioinformatics method, five signalling pathways were identified to participate in different stages of NIH3T3 cell transformation. Further, our study suggested that oncogenes , , and the tumour suppressor gene may play important roles in these pathways.
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