Induction of spermiation using Ovaprim™ with topical gill method in the silver rasbora (Rasbora argyrotaenia).

The main obstacles on silver rasbora (Rasbora argyrotaenia) culture are having the limited number of broodstock and spawning depending on the season. The purpose of this study was to determine the effect of different dosage of Ovaprim™ induction by topical gill method to silver rasbora spermiation in order to continue the production out of its reproduction season with an optimum dose. A total of 30 male fish with a weight of 7.78 ± 0.20 g and length 4.11 ± 0.31 cm was used in this research. Topical gill treatments of Ovaprim™ were administered with following doses; 0.15 μl/g, 0.25 μl/g, 0.35 μl/g, 0.45 μl/g and 0.55 μl/g body weight. Milt volume, sperm concentration, sperm motility, and sperm viability parameters were observed in this study to understand the optimum dose of Ovaprim™ for male silver rasbora breeders. Spermiation induction of silver rasbora using Ovaprim™ with topical gill method has been successfully carried out, indicating an increase (P < 0.05) in milt volume, sperm concentration, sperm motility, and sperm viability. According to results a dose of Ovaprim™ is recommended to be used the 0.25 μl/g body weight in the spermiation induction of silver rasbora.