A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 143

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 143
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 209
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 994
Function: getPubMedXML

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3134
Function: GetPubMedArticleOutput_2016

File: /var/www/html/application/controllers/Detail.php
Line: 574
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 488
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Bacterial Resistance and Prostate Cancer Susceptibility Toward Metal-Ion-doped DNA Complexes. | LitMetric

Bacterial Resistance and Prostate Cancer Susceptibility Toward Metal-Ion-doped DNA Complexes.

ACS Appl Mater Interfaces

Sungkyunkwan Advanced Institute of Nanotechnology (SAINT) and Department of Physics , Sungkyunkwan University, Suwon 16419 , Korea.

Published: December 2018

AI Article Synopsis

  • DNA nanotechnology allows the creation of custom nanoscale objects, like synthetic DNA rings and salmon-derived DNA duplexes, that can deliver metal ions (Cu, Ni, Zn) for targeting harmful bacteria and prostate cancer cells.
  • The study utilized various microscopy and spectroscopy techniques to visualize and analyze the interactions between DNA and metal ions, finding that these complexes showed no antibacterial effects but had higher cytotoxicity on cancer cells compared to the metals alone.
  • Overall, the research suggests that DNA nanostructures can serve as safe and effective nanocarriers for therapeutic delivery, enhancing the effectiveness of treatments over using the metals without DNA.

Article Abstract

DNA nanotechnology has laid a platform to construct a variety of custom-shaped nanoscale objects for functionalization of specific target materials to achieve programmability and molecular recognition. Herein, we prepared DNA nanostructures [namely, synthetic DNA rings (RDNA) and DNA duplexes extracted from salmon (SDNA)] containing metal ions (M) such as Cu, Ni, and Zn as payloads for delivery to exterminate highly pathologic hospital bacterial strains (e.g., Escherichia coli and Bacillus subtilis) and prostate cancer cells (i.e., PC3, LNCaP, TRAMP-C1, 22Rv1, and DU145). Morphologies of these M-doped RDNA were visualized using atomic force microscopy. Interactions between M and DNA were studied using UV-vis and Fourier transform infrared spectroscopy. Quantitative composition and chemical changes in DNA without or with M were obtained using X-ray photoelectron spectroscopy. In addition, M-doped DNA complexes were subjected to antibacterial activity studies. They showed no bacteriostatic or bactericidal effects on bacterial strains used. Finally, in vitro cellular toxicity study was conducted to evaluate the effect of pristine DNA and M-doped DNA complexes on prostate cancer cells. Cytotoxicities conferred by M-doped DNA complexes for most cell lines were significantly higher than those of M without DNA. Cellular uptake of these complexes was confirmed by fluorescence microscopy using PhenGreen FL indicator. On the basis of our observations, DNA nanostructures can be used as safe and efficient nanocarriers for delivery of therapeutics. They have enhanced therapeutic window than bare metals.

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.8b17013DOI Listing

Publication Analysis

Top Keywords

dna complexes
16
dna
13
prostate cancer
12
m-doped dna
12
dna nanostructures
8
bacterial strains
8
cancer cells
8
complexes
5
bacterial resistance
4
resistance prostate
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: