Antibiofilm efficacy of biosynthesized silver nanoparticles against endodontic-periodontal pathogens: An study.

Background: Endodontic-periodontal pathogens exist as biofilms which are difficult to eliminate. Biosynthesized silver nanoparticles (AgNPs) emerged as newer antimicrobial agents with potential benefits.

Aim: The aim of this study is to determine the minimum inhibitory concentration (MIC); evaluate the antibiofilm efficacy of fungal-derived AgNPs against , , and .

Materials And Methods: MIC of AgNPs against test pathogens was determined using micro broth dilution method. Serial dilutions of AgNPs ranging from 80 to 1 μg/ml concentration were added to wells containing 10 μl of bacterial inoculum in culture media and control group without AgNPs. For biofilm models, 120 dentin blocks were prepared, sterilized, and contaminated for 2 weeks with ( = 40 each). Group 1: , Group 2: , and Group 3: and each group is divided into four subgroups ( = 10 each) and treated with distilled water, AgNPs, 2% and 0.2% chlorhexidine (CHX). Colonies counted after 24 h of incubation and statistically analyzed using one-way ANOVA and Tukey tests.

Results: MIC for was determined as 20 μg/ml and 30 μg/ml for and . AgNPs were effective as 2% CHX against all biofilms compared to control group. Tukey test ( < 0.0001) shows no significant difference between groups.

Conclusion: Fungal-derived AgNPs are effective against endo-perio pathogens.