AI Article Synopsis
- The study evaluates the use of MALDI-TOF mass spectrometry as a method for identifying mycobacteria, highlighting its efficiency in routine microbiology practice due to its speed and cost-effectiveness.
- A total of 155 mycobacterial isolates, including nontuberculous mycobacteria and Mycobacterium tuberculosis complex, were analyzed for identification accuracy.
- Results showed that the MBT Mycobacteria Library 4.0 identified 100% of MTC isolates and 91% of NTM isolates accurately, proving it to be a reliable tool for mycobacterial identification.
Article Abstract
Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis.
Materials And Methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study.
Results: Identification by MBT ML4.0 was correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates.
Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3906/sag-1801-198 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!