Multilocular traits exist in a variety of plants and exert important effects on plant yield. Previous genetic studies have shown that multilocular trait of the cultivar Duoshi is controlled by two recessive genes, and . In previous studies, the gene is located on chromosome A07, and the candidate gene is . In this study, a BC4 mapping population for the gene was generated. This population was used to construct genetic linkage maps of the gene using amplified fragment length polymorphism (AFLP), intron length polymorphism (IP) and simple sequence repeat (SSR) methodology. The results showed that the gene was restricted to a 0.63 cM interval. BLAST alignment with revealed the gene was located within a 11.81-16.65 Mb region on chromosome B07. Moreover, the candidate gene (equivalent to ) was cloned by comparing mapping and map-based cloning, and gene was shown to have the ability to restore the bilocular traits in a genetic complementation experiment. The sequencing revealed that a 4961 bp insertion interrupted the coding sequence of the gene, resulting in an increase in locule number. Expression analysis revealed that was expressed in all tissues and the expression level in bilocular plants was significantly higher than that in multilocular plants. In addition, markers closely linked to the gene were developed and used for molecular marker-assisted breeding of multilocular traits.
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| http://dx.doi.org/10.3389/fpls.2018.01744 | DOI Listing |
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