Voltammetric analysis of dantrolene and its active metabolite with indomethacin in rat plasma.

Aim: Differential pulse polarography was used for the concurrent analysis of the coadministered dantrolene (DAN) and indomethacin (IND) in plasma.

Materials & Methods: DAN and IND, Hanging mercury drop electrode and Britton-Robinson buffer at pH 5 were used. In plasma, cathodic reduction of DAN nitro group and its active metabolite at -0.2 V was done. IND was analyzed after carbonyl group reduction at -1.1 V.

Results: Drugs determination in rat plasma with good recoveries and low limit of quantitation was done. Application to trace analysis of drugs in rat plasma was done with C and T determination.

Conclusion: This technique shows high sensitivity, simplicity and low cost. The method is US FDA validated and it is applicable to human level.