AI Article Synopsis
- Circular RNAs (circRNAs) were first identified 25 years ago and have been recently found across various species, showcasing their genome-wide presence.
- One gene can produce multiple circRNAs through different splicing methods, illustrating the complexity of gene expression.
- The text outlines a computational approach using the CIRCexplorer2 pipeline to accurately annotate circRNAs from existing RNA sequencing data.
Article Abstract
Circular RNAs (circRNAs) derived from back-spliced exons were sporadically identified about 25 years ago, and have been recently re-discovered genome-wide across different species. Interestingly, one gene locus can generate multiple circRNAs through alternative back-splicing and/or alternative splicing, thus expanding our understanding on the diversity and complexity of transcriptomes. Precise annotation of circRNAs with their alternative back-splicing and alternative splicing events is the basis for the functional characterization of different categories of circRNAs. Here we describe a step-by-step computational scheme to annotate circRNAs from publicly available RNA sequencing datasets with the CIRCexplorer2 pipeline.
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| http://dx.doi.org/10.1007/978-1-4939-8808-2_10 | DOI Listing |
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