AI Article Synopsis
- The text emphasizes the importance of rapidly generating monoclonal antibodies (mAbs) for research in personalized medicine and immunotherapy.
- It discusses a method using single-cell B-cell receptor cloning to create specific mAbs quickly from peripheral blood plasmablasts and memory B cells.
- The chapter also includes a detailed protocol for optimizing B-cell sorting for further analysis of B-cell specificity and function, potentially adaptable for various B-cell types and organisms.
Article Abstract
In the age of personalized medicine, an efficient method to generate monoclonal antibodies (mAbs) is essential for biomedical and immunotherapeutic research. Numerous aspects of basic B-cell biology can be studied at the monoclonal level, including B-cell development, antibody responses to infection or vaccination, and autoimmune responses. Single-cell B-cell receptor cloning allows for the rapid generation of antigen-specific mAbs in a matter of several weeks. In this chapter, we provide an efficient method to generate mAbs from peripheral blood plasmablasts and memory B cells induced by infection and vaccination. Additionally, we provide a protocol on how to optimize single-cell B-cell sorting for both single-cell B-cell receptor cloning and single-cell RNA-sequencing, for the application of studying B-cell specificity and function (spec-seq). This protocol can be easily adapted for other B-cell populations, B cells in tissues, and B cells from other organisms.
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| http://dx.doi.org/10.1007/978-1-4939-8958-4_5 | DOI Listing |
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