Endothelial cell culture under flow, to mimic physiological conditions within blood vessels, has gained particular attention for the formation of a homogeneous endothelium in vitro. Here, we report on the design of a setup for simultaneous culture of up to nine electrospun membranes or thin polymer films in custom-made holders under flow on an orbital shaker. The versatile design of the device allows for the use of electrospun membranes/polymer films of choice and subsequent analysis with commonly used methods such as immunofluorescence or scanning electron microscopy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/7651_2018_192 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
2D Titanium Carbide MXene-Interfaced Zinc Oxide/Tungstite Architectures Adorned Mixed Matrix Polymer Membranes for Oily Wastewater Treatment.
ACS Appl Mater Interfaces
January 2025
Department of Physics, National Institute of Technology Nagaland, Chumukedima, Dimapur 797103, India.
An exceedingly porous and interwoven fibrous structure was achieved in this study by interlocking titanium carbide (TiC) MXenes onto the electrospun mats using poly(vinylidene fluoride) (PVDF) as the base polymer. The fibrous membrane was further modified with the inclusion of zinc oxide (ZnO) and tungstite (WO·HO) nano/microstructures via annealing and hydrothermal approaches. Through these strategic interfaced morphological developments in novel TiC/ZnO/WO·HO heterostructures, our findings reveal enhanced wettability and charge-segregation desirable for promoting oil-water separation and photoreactivity, respectively.
View Article and Find Full Text PDFEngineered Lubricative Lecithin-Based Electrospun Nanofibers for the Prevention of Postoperative Abdominal Adhesion.
Pharmaceutics
December 2024
Basic Research Key Laboratory of General Surgery for Digital Medicine, Affiliated Hospital of Hebei University, Baoding 071000, China.
: Postoperative abdominal adhesion is a prevalent complication following abdominal surgery, with the incidence of adhesion reaching up to 90%, which may precipitate a range of adverse outcomes. Although fibrous membranes loaded with various anti-inflammatory or other drugs have been proposed for anti-adhesion, most of them suffer from drug-induced adverse effects. : In this study, a lecithin-based electrospun polylactic acid (PLA) nanofibrous membrane (L/P-NM) was developed for the prevention of postoperative abdominal adhesion, utilizing the hydration lubrication theory.
View Article and Find Full Text PDFPreparation and Characterization of Highly Conductive PVDF/PAN Conjugate Electrospun Fibrous Membranes with Embedded Silver Nanoparticles.
Polymers (Basel)
December 2024
College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China.
This study reports the development of highly conductive and stretchable fibrous membranes based on PVDF/PAN conjugate electrospinning with embedded silver nanoparticles (AgNPs) for wearable sensing applications. The fabrication process integrated conjugate electrospinning of PVDF/PAN, selective dissolution of polyvinylpyrrolidone (PVP) to create porous networks, and uniform AgNP incorporation via adsorption-reduction. Systematic optimization revealed that 10 wt.
View Article and Find Full Text PDFFlexible PAN/P25 Multi-Porous Nanotubular Electrospun Membrane Constructed by a Facile Ethylene Glycol Solvothermal Induction with Excellent Photocatalytic Degradation and Sterilization Performance.
Polymers (Basel)
December 2024
Hubei Three Gorges Laboratory, Yichang 443000, China.
A series of flexible polyacrylonitrile/TiO (PAN/P25) multi-porous nanotubular membranes were successfully constructed by facile electrospinning combined with an ethylene glycol solvothermal induce strategy. The effects of P25 dosage and solvothermal time on the morphology of samples were systematically investigated, which were characterized in terms of surface morphology, microstructure, specific surface area, thermal analysis, wettability, photoelectrochemical and fluorescence spectra. Rhodamine B (RhB) and () were employed as simulated pollutants to evaluate photocatalytic degradation and antibacterial properties of the PAN/P25-3 multi-porous nanotubular membrane.
View Article and Find Full Text PDFPolylactic Acid/Bamboo Leaf Extract Electrospun Mats with Antioxidant Activity for Food Packaging Applications.
Antioxidants (Basel)
December 2024
Department of Engineering, University of Palermo, Viale delle Scienze, 90128 Palermo, Italy.
This study focuses on developing an active and biodegradable packaging using electrospinning, with polylactic acid (PLA) as the matrix and bamboo leaf extract (BLE) as the antioxidant compound. The research systematically evaluates the relationship among process parameters, material properties, and structure. The electrospun membranes were produced using different BLE contents (10 wt%, 20 wt%, 30 wt%, and 40 wt%) and characterized by their morphology, mechanical properties, wettability, and antioxidant activity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!