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Differentiation of and by Metabolite Profiles Obtained Using Gold Nanoparticles-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry.
Pathogens
December 2024
Department of Inorganic and Analytical Chemistry, Faculty of Chemistry, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland.
and are challenging to differentiate using methods such as phenotyping, 16S rRNA sequencing, or protein profiling through matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) due to their close relatedness. This study explores the potential for identifying and by incorporating reference spectra of metabolite profiles, obtained via surface-assisted laser desorption/ionization mass spectrometry (SALDI MS) employing gold nanoparticles (AuNPs), into the Bruker Biotyper database. Metabolite extracts from and cells were prepared using liquid-liquid extraction in a chloroform-methanol-water system.
View Article and Find Full Text PDFAntimicrobial Effectiveness of Clove Oil in Decontamination of Ready-to-Eat Spinach ( L.).
Foods
January 2025
College of Agriculture, Science and Technology, Delaware State University, 1200 North DuPont Highway, Dover, DE 19901, USA.
Due to an increased demand for natural food additives, clove oil was assessed as a natural alternative to chemical disinfectants in produce washing. This study assessed the antimicrobial activity of 5 and 10% (/) clove oil-amended wash liquid (CO) using a zone of inhibition (ZIB) test and determined the time required to completely inactivate pathogenic bacteria using bacterial death curve analysis. A washing experiment was used to evaluate CO's ability to inhibit bacterial growth on inoculated RTE spinach and in the wash water.
View Article and Find Full Text PDFand infection in led to species-specific regulatory responses in the host and pathogen.
Microb Genom
January 2025
School of Science, Monash University Malaysia, 47500 Bandar Sunway, Selangor Darul Ehsan, Subang Jaya, Malaysia.
In recent decades, has surpassed as the leading cause of shigellosis, possibly due to species-specific differences in their transcriptomic responses. This study used dual RNA sequencing to analyse the transcriptomic responses of and the two species at early (10 minutes) and late (24 hours) stages of infection. While the nematode defence response was downregulated during both infections, only infection by led to downregulation of sphingolipid metabolism, cadmium ion response and xenobiotic response in .
View Article and Find Full Text PDFOccurrence of Antibiotic-Resistant Bacteria in Urban Surface Water Sources in Bangladesh.
Curr Microbiol
January 2025
Fisheries and Marine Resources Technology Discipline, Khulna University, Khulna, 9208, Bangladesh.
Infections caused by antibiotic-resistant bacteria (ARB) result in an estimated 1.27 million human deaths annually worldwide. Surface waters are impacted by anthropogenic factors, which contribute to the emergence and spread of ARB in the aquatic environment.
View Article and Find Full Text PDFMolecular epidemiology of extended-spectrum beta-lactamases and carbapenemases-producing Shigella in Africa: a systematic review and meta-analysis.
BMC Infect Dis
January 2025
Department of Medical Microbiology, University of Ghana Medical School, Korle Bu, P.O. Box KB 4236, Accra, Ghana.
Background: The treatment of Shigella infections has become a major challenge due to the emergence of multidrug-resistant Shigella. There is however insufficient knowledge regarding the molecular epidemiology of Shigella strains producing beta-lactamases in Africa. This systematic review investigated the scientific literature on the molecular epidemiology of extended-spectrum beta-lactamase (ESBL) and carbapenemases producing Shigella in Africa.
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