Objective: Different effects of microRNA-495 (miR-495) on human cancers have been exhibited in recent years. However, the specific function of miR-495 remains uncertain in non-small-cell lung cancer (NSCLC). Thus, we aim to explore the role of miR-495 in NSCLC.
Patients And Methods: The expressions of miR-495 and transcription factor 4 (TCF4) were detected through quantitative Real-time polymerase chain reaction (qRT-PCR) assay. Western blot was used to measure the protein expression of relative genes. The relationship between miR-495 and TCF4 was testified by the dual-luciferase reporter gene assay. The function of miR-495 was investigated through cell counting kit-8 (CCK-8) assay and transwell assay.
Results: MiR-495 was downregulated in NSCLC tissues. Overexpression of miR-495 inhibited the migration, invasion and proliferation of NSCLC cells. Further, TCF4 was a direct target gene of miR-495. TCF4 was highly expressed in NSCLC tissues. In addition, miR-495 inhibited the progression of NSCLC through targeting TCF4. Furthermore, miR-495 inhibited EMT and Wnt/β-catenin pathway in NSCLC.
Conclusions: MiR-495 inhibited the progression of NSCLC by targeting TCF4 and inactivating Wnt/β-catenin pathway.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.26355/eurrev_201811_16398 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Maternal exercise programs placental miR-495-5p-mediated Snx7 expression and kynurenic acid metabolic pathway induced by prenatal high-fat diet: Based on miRNA-seq, transcriptomics, and metabolomics.
J Nutr Biochem
December 2024
Key Laboratory of Endocrinology of National Health Commission, Diabetes Research Center of Chinese Academy of Medical Sciences, Department of Endocrinology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100730, China. Electronic address:
Poor intrauterine environments increase the prevalence of chronic metabolic diseases in offspring, whereas maternal exercise is an effective measure to break this vicious intergenerational cycle. Placenta is increasingly being studied to explore its role in maternal-fetal metabolic cross-talk. The association between placental miRNA and offspring development trajectories has been established, yet the specific role and mechanism thereof in maternal exercise-induced metabolic protection remain elusive.
View Article and Find Full Text PDFGAS5 promotes glucose metabolism reprogramming and resistance to ferroptosis of endothelial progenitor cells through the miR-495-3p/SIX1 and IGF2BP2/NRF2 dual-regulatory pathways in coronary heart disease.
Cell Mol Biol (Noisy-le-grand)
October 2024
Department of Cardiology, Heart Center, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, China.
Article Synopsis
- The study investigates the role of lncRNA GAS5 in regulating glucose metabolism and ferroptosis in endothelial progenitor cells (EPCs) amidst coronary heart disease (CHD).
- Researchers used various assays to analyze cell behavior, glucose uptake, and markers of ferroptosis, finding GAS5 levels were decreased in CHD patients compared to healthy individuals.
- Results indicate that GAS5 depletion negatively impacts EPC proliferation, migration, and increases apoptosis, while GAS5 enhances glucose metabolism and protects against ferroptosis by influencing the NRF2 and SIX1 pathways.
miR-495 promotes intestinal epithelial cell apoptosis through downregulation of Sphingosine-1-phosphate.
Physiol Rep
September 2024
Baltimore Veterans Affairs Medical Center, Baltimore, Maryland, USA.
Many pathological conditions lead to defects in intestinal epithelial integrity and loss of barrier function; Sphingosine-1-phosphate (S1P) has been shown to augment intestinal barrier integrity, though the exact mechanisms are not completely understood. We have previously shown that overexpression of Sphingosine Kinase 1 (SphK1), the rate limiting enzyme for S1P synthesis, significantly increased S1P production and cell proliferation. Here we show that microRNA 495 (miR-495) upregulation led to decreased levels of SphK1 resultant from a direct effect at the SphK1 mRNA.
View Article and Find Full Text PDFBaitouweng decoction suppresses growth of esophageal carcinoma cells through miR-495-3p/BUB1/STAT3 axis.
World J Gastrointest Oncol
July 2024
Henan Key Laboratory of Traditional Chinese Medicine Syndrome and Prescription in Signaling, Henan International Joint Laboratory of TCM Syndrome and Prescription in Signaling, Traditional Chinese Medicine School, Henan University of Chinese Medicine, Zhengzhou 450046, Henan Province, China.
Background: Esophageal carcinoma (EC) is one of the most prevalent cancers in human populations worldwide. Baitouweng decoction is one of the most important Chinese medicine formulas, with the potential to treat cancer.
Aim: To investigate the role and mechanism of Baitouweng decoction on EC cells.
Terpenoids from quinoa reverse drug resistance of colon cancer by upregulating miR-495-3p.
J Sci Food Agric
November 2024
Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China.
Background: Quinoa contains far more nutrients than any traditional grain crop. It is known that terpenoids in quinoa have anti-inflammatory and antitumor effects, but their role in reversing drug resistance remains unclear.
Results: Our previous studies showed that quinoa-derived terpenoid compounds (QBT) can inhibit the occurrence and development of colon cancer.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!