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Multiscale Three-Dimensional Evaluation and Analysis of Murine Lung Vasculature From Macro- to Micro-Structural Level.
Pulm Circ
January 2025
Department of Imaging and Pathology, Biomedical MRI KU Leuven Leuven Belgium.
The pulmonary vasculature plays a pivotal role in the development and progress of chronic lung diseases. Due to limitations of conventional two-dimensional histological methods, the complexity and the detailed anatomy of the lung blood circulation might be overlooked. In this study, we demonstrate the practical use of optical serial block face imaging (SBFI), ex vivo microcomputed tomography (micro-CT), and nondestructive optical tomography for visualization and quantification of the pulmonary circulation's 3D architecture from macro- to micro-structural levels in murine lung samples.
View Article and Find Full Text PDFCryopreservation of brain cell structure: a review.
Free Neuropathol
January 2024
Friedman Brain Institute, Departments of Pathology, Neuroscience, and Artificial Intelligence & Human Health, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Cryopreservation, the preservation of tissues at subzero temperatures, is a mainstay of brain banking that allows for the storage of brain tissue without the use of chemical fixatives. This is particularly important for molecular studies that are incompatible with tissue fixation. However, brain tissue is vulnerable to various forms of damage during the cryopreservation process, in particular due to the phase transition of water from a liquid to a solid state with the formation of ice crystals, which can disrupt cellular morphology.
View Article and Find Full Text PDFNanoscale interaction mechanism between bubbles and microplastics under the influence of natural organic matter in simulated marine environment.
J Hazard Mater
January 2025
School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, PR China. Electronic address:
Bubble-microplastic (MP) interaction is a significant process that changes the routes of MP circulation in marine environment and thereby determines the risk of MPs, which could be strongly influenced by natural organic matter (NOM) in oceans. However, the quantitative interaction mechanisms between bubbles and MPs under the effect of NOM remain elusive. Herein, bubble-MP interactions in simulated seawater were quantified at nanoscale based on atomic force microscope coupled with the Stokes-Reynold-Young-Laplace model.
View Article and Find Full Text PDFA Novel Polarized Light Microscope for the Examination of Birefringent Crystals in Synovial Fluid.
Gout Urate Cryst Depos Dis
December 2024
Electrical and Computer Engineering Department, University of California, Los Angeles, CA 90095, USA.
Background: The gold standard for crystal arthritis diagnosis relies on the identification of either monosodium urate (MSU) or calcium pyrophosphate (CPP) crystals in synovial fluid. With the goal of enhanced crystal detection, we adapted a standard compensated polarized light microscope (CPLM) with a polarized digital camera and multi-focal depth imaging capabilities to create digital images from synovial fluid mounted on microscope slides. Using this single-shot computational polarized light microscopy (SCPLM) method, we compared rates of crystal detection and raters' preference for image.
View Article and Find Full Text PDF[Physiological characteristics of subspecies with double mutations of and and its activity against larvae of ].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
December 2024
Lixiahe Institute of Agricultural Sciences in Jiangsu Province; National Experimental Station of Yangzhou for Agricultural Microbiology, Yangzhou, Jiangsu 225007, China.
Objective: To investigate the physiological characteristics of subspecies (Bti) with double mutations of and genes and to assess the activity of Bti against larvae of under different external factors, so as to provide the theoretical evidence for the use of engineered bacteria of Bti for effective mosquito control.
Methods: wild-type strain Bt-59 and Bt-59 strain with mutation [Bt-59 (Δ)] were cultured in nutrient broth media for 24 hours, and Bt-59 strains with mutation [Bt-59 (Δ)] and double mutations of and [Bt-59 (Δ)] were cultured in nutrient broth media for 48 hours. Then, 5 μL of culture media were transferred to glass sides, and cell morphology and mother cell lysis were observed under an optical microscope.
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