AI Article Synopsis
- Aurantiochytrium limacinum is recognized for its high levels of polyunsaturated fatty acids, specifically docosahexaenoic acid (DHA), which is produced via the polyketide synthase (PKS) pathway involving three gene clusters: pks1, pks2, and pks3.
- The researchers successfully amplified the full-length pks3 gene and identified key enzyme domains (KR and DH) that are important for DHA synthesis.
- By knocking in the KR and DH genes into A. limacinum, they increased the total fatty acid content and unsaturation levels, paving the way for enhanced industrial production of DHA.
Article Abstract
Aurantiochytrium limacinum has received attention because of its abundance of polyunsaturated fatty acids (PUFAs), particularly docosahexaenoic acid (DHA). DHA is synthesized through the polyketide synthase (PKS) pathway in A. limacinum. The related enzymes of the PKS pathway are mainly expressed by three gene clusters, called pks1, pks2 and pks3. In this study, the full-length pks3 gene was obtained by polymerase chain reaction amplification and Genome Walking technology. Based on a domain analysis of the deduced amino acid sequence of the pks3 gene, 3-ketoacyl-ACP reductase (KR) and dehydratase (DH) enzyme domains were identified. Herein, A. limacinum OUC168 was engineered by gene knock-in of KR and DH using the 18S rDNA sequence as the homologous recombination site. Total fatty acid contents and the degree of unsaturation of total fatty acids increased after the kr or dh gene was knocked in. The cloning and functional study of the pks3 gene of A. limacinum establishes a foundation for revealing the DHA synthetic pathway. Gene knock-in of the enzyme domain associated with PKS synthesis has the potential to provide effective recombinant strains with higher DHA content for industrial applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289434 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208853 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Final piece to the Fusarium pigmentation puzzle - Unraveling of the phenalenone biosynthetic pathway responsible for perithecial pigmentation in the Fusarium solani species complex.
Fungal Genet Biol
September 2024
Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8A, 6700 Esbjerg, Denmark. Electronic address:
The Fusarium solani species complex (FSSC) is comprised of important pathogens of plants and humans. A distinctive feature of FSSC species is perithecial pigmentation. While the dark perithecial pigments of other Fusarium species are derived from fusarubins synthesized by polyketide synthase 3 (PKS3), the perithecial pigments of FSSC are derived from an unknown metabolite synthesized by PKS35.
View Article and Find Full Text PDFTransboundary milRNAs: Indispensable molecules in the process of Trichoderma breve T069 mycoparasitism of Botrytis cinerea.
Pestic Biochem Physiol
November 2023
Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests (Hainan University), Ministry of Education, Haikou 570228, China. Electronic address:
Article Synopsis
- The study assesses the role of fungal microRNA-like small RNAs (milRNAs) in the mycoparasitism process of Trichoderma breve T069 against the pathogenic fungus Botrytis cinerea.
- Researchers identified different stages of mycoparasitism, revealing distinct behaviors at 12, 36, and 72 hours post hyphal contact.
- High-throughput sequencing identified numerous milRNAs from T. breve T069, which may target genes in B. cinerea, highlighting the potential for these milRNAs to influence fungal interactions and pathogenicity.
An Unconventional Melanin Biosynthesis Pathway in Ustilago maydis.
Appl Environ Microbiol
January 2021
Department of Biology, Philipps University Marburg, Marburg, Germany
is a phytopathogenic fungus responsible for corn smut disease. Although it is a very well-established model organism for the study of plant-microbe interactions, its potential to produce specialized metabolites, which might contribute to this interaction, has not been studied in detail. By analyzing the genome, we identified a biosynthetic gene cluster whose activation led to the production of a black melanin pigment.
View Article and Find Full Text PDFHeterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of .
Int J Mol Sci
October 2020
Aalborg University Esbjerg, Department of Chemistry and Bioscience, Niels Bohrs Vej 8, 6700 Esbjerg, Denmark.
Article Synopsis
- Researchers recreated a biosynthetic gene cluster to produce bostrycoidin, a red pigment compound!
- They used a method called sequential transformation associated recombination (TAR) cloning in a specific vector system to express the necessary genes!
- After testing in growth cultures, they found the highest production of bostrycoidin (2.2 mg/L) occurred after 2 days of inducing the process!
A new vector system for targeted integration and overexpression of genes in the crop pathogen .
Fungal Biol Biotechnol
December 2019
1Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8, 6700 Esbjerg, Denmark.
Background: Besides their ability to produce several interesting bioactive secondary metabolites, members of the species complex comprise important pathogens of plants and humans. One of the major obstacles in understanding the biology of this species complex is the lack of efficient molecular tools for genetic manipulation.
Results: To remove this obstacle we here report the development of a reliable system where the vectors are generated through yeast recombinational cloning and inserted into a specific site in through -mediated transformation.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!