Identification and characterization of a novel antimicrobial peptide compound produced by strain isolated from oral microflora.

In recent years, the decreased efficacy of existing antibiotics toward management of emergent drug-resistant strains has necessitated the search for novel antibiotics from natural products. In this regard, sp is well known for producing variety of secondary metabolites of potential use. Therefore, we performed an investigation to isolate and identify sp from oral cavity for production of novel antimicrobial compounds. We extracted, purified, and identified a novel bioactive compound by (KC246043.1). The optimal production of compound was observed on de Man Rogosa and Sharpe broth by incubating at 37 °C, and pH 7.0 for 4 days. The bioactive compound was extracted by using -butanol (2:1 v/v), purified on TLC plates with detection at R 7.8 cm; further characterized and identified as a cyclic ploypeptide sharing structural similarity with bacitracin. Minimum inhibitory concentration of bioactive compound was found to be 0.25, 0.5, 1.0, 3.125 and 6.25 μg/ml against ATCC10240, ATCC19430, ATCC35218. ATCC27853 and ATCC25923 respectively, with no activity against ATCC10231. Our findings have revealed a novel cyclic peptide compound from with broad spectrum antimicrobial activity against both Gram positive and Gram negative bacteria.