[Role of TGFβ1 and VEGF-А gene polymorphisms in development of chronic obliterating diseases of lower-limb arteries].

The study was aimed at investigating the interrelationship of gene polymorphisms of transforming growth factor (TGFβ1) and endothelial vascular growth factor (VEGF-A) with the risk for the development of chronic obliterating diseases of lower-limb arteries (CODLLA) in the Russian population of Central Russia. We studied polymorphism C-509T of the TGFβ1 gene and polymorphism +936C>T of the VEGF-A gene in 202 patients with CODLLA and in 148 apparently healthy individuals. Genomic DNA was isolated by the standard method of phenol-chloroform extraction. These polymorphisms were genotyped by means of polymerase chain reaction followed by analysing restriction fragments length polymorphism. Patients with CODLLA were found to have a decrease of frequency of homozygous genotypes of transforming growth factor β1 for the wild-type alleles: -509CC (p=0.02) and an increase of frequency of homozygotes for the variant allele: -509TT (p=0.00004), which was indicative of an abrupt increase in secretion of transforming growth factor. We revealed no deviation of associations of the examined polymorphisms by the significant homozygous carrier state for the normal allele +936CC of endothelial growth factor gene as compared with the healthy cohort. A conclusion was made that a proatherogenic risk factor for CODLLA is an increase of TGFβ1 secretion, with no genetically-determined prerequisites of the effect of VEGF-A gene polymorphism on the development of the disease revealed.