AI Article Synopsis
- The study aimed to assess a new method for the early diagnosis of tuberculosis using a specific assay.
- A total of 374 samples from patients with suspected TB were tested, resulting in 30 strains of Mycobacterium tuberculosis being identified, all sensitive to rifampicin.
- The new testing method demonstrated 100% accuracy in sensitivity, specificity, and predictive values when compared to traditional culture methods, potentially speeding up TB diagnosis and treatment adjustments.
Article Abstract
Objectives: To evaluate a new tool for the early diagnosis of tuberculosis.
Methods: A total of 374 smear-negative clinical specimens from patients with suspected tuberculosis were evaluated using a new procedure consisting of a preliminary step of culture in broth bottles followed by the detection of Mycobacterium tuberculosis complex (Mtb) and rifampicin resistance by the Xpert MTB/RIF assay (XMTB-RIF).
Results: A total of 30 Mtb strains were isolated, all susceptible to rifampicin. When broth cultures were subjected to XMTB-RIF analysis after 15 days of incubation, sensitivity, specificity, PPV, and NPV were each 100% when compared with liquid culture.
Conclusion: The XMTB-RIF assay used in 15-day broth cultures may provide a final culture result for smear-negative specimens. This process, combined with clinical signs, may contribute to rapidly diagnosing tuberculosis and also to the early reevaluation of empirical antituberculosis treatment.
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| http://dx.doi.org/10.1016/j.medmal.2018.11.004 | DOI Listing |
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