AI Article Synopsis
- The study showcases the use of stimulated emission depletion (STED) microscopy for imaging whole bacterial and eukaryotic cells with reversible fluorogenic labels.
- By constantly exchanging these labels, the method prevents the common problem of photobleaching, allowing for improved imaging quality.
- The technique enables long acquisition times and supports 3D, multicolor, and live-cell imaging of entire cells.
Article Abstract
We demonstrate stimulated emission depletion (STED) microscopy of whole bacterial and eukaryotic cells using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging. We achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multicolor, and live-cell STED microscopy.
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| http://dx.doi.org/10.1021/acs.nanolett.8b04385 | DOI Listing |
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