AI Article Synopsis
- The text discusses a well-studied fungus known for producing penicillin, highlighting its significance in antibiotic production since penicillin's discovery.
- Various strain improvement programs have increased penicillin output significantly but have suppressed the production of other natural products due to gene silencing.
- The text also explores methods to reactivate the suppressed biosynthetic gene clusters (BGCs) and discusses the potential of protein engineering to enhance the fungus’s ability to produce new natural products for commercial use.
Article Abstract
(renamed ) is the most studied member of a family of more than 350 species that constitute the genus. Since the discovery of penicillin by Alexander Fleming, this filamentous fungus is used as a commercial β-lactam antibiotic producer. For several decades, was subjected to a classical strain improvement (CSI) program to increase penicillin titers. This resulted in a massive increase in the penicillin production capacity, paralleled by the silencing of several other biosynthetic gene clusters (BGCs), causing a reduction in the production of a broad range of BGC encoded natural products (NPs). Several approaches have been used to restore the ability of the penicillin production strains to synthetize the NPs lost during the CSI. Here, we summarize various re-activation mechanisms of BGCs, and how interference with regulation can be used as a strategy to activate or silence BGCs in filamentous fungi. To further emphasize the versatility of as a fungal production platform for NPs with potential commercial value, protein engineering of biosynthetic enzymes is discussed as a tool to develop BGC pathways for new NPs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262359 | PMC |
| http://dx.doi.org/10.3389/fmicb.2018.02768 | DOI Listing |
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