AI Article Synopsis
- Feline panleukopenia virus (FPV) is a deadly pathogen primarily affecting kittens, and it's linked to canine parvovirus, making it significant for research in the Parvoviridae family.
- Researchers developed a full-length infectious clone of FPV strain HH-1/86, which has a 5123-nucleotide single-stranded DNA genome with distinctive inverted terminal repeats.
- The study successfully transfected feline kidney cells with this clone, leading to the recovery of a virus (rFPV) that closely mirrored the original virus, enabling further research on its pathogenicity, replication, and potential transmission between species.
Article Abstract
Feline panleukopenia virus (FPV) infects cats and can be fatal to kittens. There is evidence that canine parvovirus originated from FPV, which makes FPV important in studies of the family Parvoviridae. In the present study, the entire genome of FPV strain HH-1/86 was converted into a full-length infectious clone (pFPV). The FPV strain HH-1/86 has a 5123-nt single stranded DNA genome with a Y-shaped inverted 3' terminal repeat (ITR) and a U-shaped inverted 5' ITR. Feline kidney cells (F81) were transfected with the pFPV clone which contained a genetic marker, and a rescued virus was obtained (rFPV). The rFPV was identified by its cytopathic effects, indirect immunofluorescence, growth curve analysis, western blot assay and hemagglutination, and was indistinguishable from the parent virus. The FPV infectious clone will facilitate the study of pathogenicity and viral replication of FPV and the inter-species transmission of parvoviruses.
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