AI Article Synopsis
- Microcapsules made from alginate-keratin and different formulations were created and analyzed using various microscopy techniques and spectroscopy.
- The ADA-based materials showed a higher degradation rate than standard alginate materials, and adding bioactive glass (BG) enhanced the bioactivity of the microcapsules.
- In tests with MG-63 osteosarcoma cells, while BG had an initial negative impact on cell growth, over time, the formation of calcium phosphate (CaP) suggested long-term benefits from using BG in cell-laden bioinks for biofabrication.
Article Abstract
Microcapsules based on alginate-keratin, alginate dialdehyde (ADA)-keratin and ADA-keratin-45S5 bioactive glass (BG) were successfully prepared. The samples were characterized by light microscopy, scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The results showed that ADA-based materials possess higher degradation rate compared to alginate-based materials. The incorporation of BG particles (mean particle size: 2.0 µm) improved the bioactivity of the materials. Moreover, the biological properties of the samples were evaluated by encapsulating MG-63 osteosarcoma cells into the microcapsules. The cell viability in all samples increased during 21 days of cultivation. However, the presence of 0.5% BG particle seemed to have initial negative effect on cell growth compared to other samples without BG. On the other hand, the positive effect of CaP formation was visible after 3 weeks in the BG containing samples. The results are relevant to consider the development of cell laden bioinks incorporating inorganic bioactive particles for biofabrication approaches.
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| http://dx.doi.org/10.1007/s10856-018-6195-5 | DOI Listing |
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